Search results for "Anisakis pegreffii"
showing 3 items of 3 documents
Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines
2016
Background In countries with elevated prevalence of zoonotic anisakiasis and high awareness of this parasitosis, a considerable number of cases that associate Anisakis sp. (Nematoda, Anisakidae) and different bowel carcinomas have been described. Although neoplasia and embedded larvae were observed sharing the common site affected by chronic inflammation, no association between the nematode and malignancy were directly proved. Similarly, no data are available about the effect of secretory and excretory products of infecting larvae at the host’s cellular level, except in respect to allergenic interaction. Methods To test the mechanisms by which human non-immune cells respond to the larvae, w…
Seasonal trend of Anisakidae infestation in South Mediterranean bluefish
2020
A total of 1104 fish samples from markets of Sicily were analysed for the detection and species identification of Anisakidae nematodes. The preliminary analysis of the fish samples showed the presence of 2459 larvae. All the fish species revealed different prevalence of infestation, with a maximum of 100% for Lepidopus caudatus and a minimum of 4.5% in Sardina pilchardus. The 80% of the larvae examined by PCR-RFLP analysis belonged to Anisakis pegreffii species. The seasonal infestation trend of Anisakis was evaluated in all the fish sample examined. The results of the seasonal infestation trend showed a marked connection with the ecological aspects of the fish species examined. As far as w…
On the morphology of Anisakis pegreffii: a comparative analysis of three microscopic techniques used to build a new parasite atlas
2014
BACKGROUND: Human anisakidosis is a parasitic anthropozoonosis caused by larval nematodes of the family Anisakidae. Here, we report a detailed description of the morphology of Anisakis pegreffii third-stage larva performed using a conventional light and confocal microscopy, and scanning electron microscopy (SEM) that provide a basis for both phenotypic studies and genetic mutations. METHODS: The collected larvae from fish were morphologically identified as Anisakis larvae Type I, and they were characterized by PCR-RFLP to identify the Anisakis pegreffii specie. Using NC5/NC2 primers, ribosomal genomic regions ITS1, 5.8 SrRNA and ITS2 of DNA were amplified and PCR products were sequenced. Fi…